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Formation of biofilm by probiotic bacteria and its processing to solid drug form.
Grossová, Marie ; Rittich, Bohuslav (referee) ; Buňka, František (referee) ; Márová, Ivana (advisor)
The aim of present work is cultivation of probiotic bacteria L. acidophilus, B. breve and B. longum in such a way that the culture forms cells clusters or comprehensive biofilm on the variety of free carriers. Biofilm formation of L. acidophilus on the silica from point of view bile and acid tolerance in gastrointestinal tract was studied. While the number of living cells in planktonic form (planktonic form) at pH 1 fell by 30 %, the viability of the biofilm cells was maintained to 90 % under the same environmental conditions. The biofilm culture showed also the protection against environment contained bile. Furthermore, the possibilities of drying procedures of biofilm cultures used as commercial technologies in pharmaceutical industry were studied. The comparison of freeze-drying and fluidization bed drying showed, that freeze-drying is more suitable method, which is able to achieve higher amount of viable cells after drying than fluidization bed drying. The effectivity of freeze-drying method is dependent on the selection of suitable cryprotective medium. In this case, about 90 % higher viability after freeze drying was achieved in comparison with fluidization bed drying. Finally, the industrial processing of probiotic strains into the solid dosage form was studied. Tablets should be produced at hardness between 70 and 90 N and water activity of tablet mixture can be maintained below 0.3. Consequently, the drying step of the tablets in a hermetically closed space with at least 10 % of silica gel must be ensured. Thereafter, the tablets contain (5.4 ± 0.7)109 viable cells after 6 months of drying process. Capsule production technology has no significant effect on the cell‘s viability during production. The triplex blistering foil for primary blistering of probiotic capsules was chosen. The triplex foil, which has low values of water vapour transition rate (0.07 g H2O / (m2 × day) and oxygen transition rate (0.01 cm3/m2 × day), was chosen. Other studied blistering foils commonly used in the pharmaceutical industry are not suitable for long storage of solid dosage forms contained probiotics.
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Moulds and residential buildings
Dvořák, Tomáš ; Trachtová, Štěpánka (referee) ; Omelková, Jiřina (advisor)
Bachelor’s thesis is aimed at moulds and residential buildings. At moulds occurrence and at conditions for their growth and reproduction. Thesis defined their distribution, morphology, metabolism and type of reproduction. Describe most important genera of moulds, their identification and procedure during their elimination. Practical part is aimed at two methods of determination in two different types of residential building, in prefab house and in single family house. The object of practical part was to determinate the genus of occurring moulds
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Quality parameters of dry fermented sausages
Pavlík, Zdeněk ; Ing.Miroslav Jůzl, Ph.D. (referee) ; Alena,, Saláková (advisor)
The aim of this study was to evaluate quality parameters of dry fermented sausages. Samples of two types of dry fermented sausages were sampled – sausage “Poličan“ and “Lovecký salám“, from three different producers. Basic physical-chemical analysis of quality parameters were performed in sausage mixture and immediately after end of ripening (21 days for Poličan and 16 days for Lovecký salám). Results of quality parameters analysis were compared with requirements of Czech legislation and differences between producers were observed. There were significant differences between producers, however all of them met requirements set by 326/2001 Czech notification as amended of Ministry of agricultural.
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The influence of water activity on the microbiological content in meat products
BURIANOVÁ, Michaela
The aim of this thesis is to monitor how the amount of water (water activity) affects the amount of microorganisms is selected packaged meat products from the point of view standards, which specify their specific requirements for the health safety of these products. The first part of this work describes various types of packaging materials of meat products, the microorganisms that occur in them and the definition of water activity. The second part describes the actual measurement of water activity and the measurement of the occurrence of microorganisms and coliforms, from selection of suitable meat products, their types of packaging, sample preparation and evaluation of measured methods. The results showed, that the activity of water affects the occurrence of microorganisms and coliforms, in that the higher the activity of water, the higher microorganisms presence.
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Morphological indications of water activity on Mars
Klíma, Jan ; Kalvoda, Jan (advisor) ; Šobr, Miroslav (referee)
Morphological indications of water activity on Mars Bachelor thesis deals with morphological indications of water activity on the Mars surface. In the papers are summarized and characterized the structures and forms on the surface of planet Mars, which originated in the past by water in gaseous, liquid or solid form. Based on research articles and expert studies, a bachelor thesis has been composed. It provides the summary of shapes on the Mars surface, which were created by water activity. There are many water formed parts of the Martian surface nowadays: valley networks, an extensive basin in the northern hemisphere, which is the remnant of antient ocean, craters lakes in the past, water bound as a solid component of polar caps and many others. The development of water-formed shapes of the Martian surface is described, and these shapes are also located on a brief map. In presented study is elaborated a comprehensive and complex view to morphological indications of the water activity on Mars.
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Mechanisms of water stress adaptations of the filamentous fungi
Sklenář, František ; Hubka, Vít (advisor) ; Nováková, Alena (referee)
Some species of the filamentous fungi can grow on the substrates with low water activity that have either low water content or high concentration of osmotically active substances. These fungi are usually called osmotolerant or xerotolerant. They occur particularly in the soil of arid areas and in hypersaline environments. Economically important species cause spoilage of stored food, seed and feed. This thesis brings the summary of physiological and morphological adaptations of fungal organisms to water stress and deals with the molecular genetic background of these adaptations. The current taxonomic classification of the fungal organisms adapted to grow at low water activity is summarized. Separate chapters are dedicated to the influence of different osmotically active substances on the phenotype of the fungi and to the possibilities of using media with osmotically active substances for taxonomic purposes.
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Formation of biofilm by probiotic bacteria and its processing to solid drug form.
Grossová, Marie ; Rittich, Bohuslav (referee) ; Buňka, František (referee) ; Márová, Ivana (advisor)
The aim of present work is cultivation of probiotic bacteria L. acidophilus, B. breve and B. longum in such a way that the culture forms cells clusters or comprehensive biofilm on the variety of free carriers. Biofilm formation of L. acidophilus on the silica from point of view bile and acid tolerance in gastrointestinal tract was studied. While the number of living cells in planktonic form (planktonic form) at pH 1 fell by 30 %, the viability of the biofilm cells was maintained to 90 % under the same environmental conditions. The biofilm culture showed also the protection against environment contained bile. Furthermore, the possibilities of drying procedures of biofilm cultures used as commercial technologies in pharmaceutical industry were studied. The comparison of freeze-drying and fluidization bed drying showed, that freeze-drying is more suitable method, which is able to achieve higher amount of viable cells after drying than fluidization bed drying. The effectivity of freeze-drying method is dependent on the selection of suitable cryprotective medium. In this case, about 90 % higher viability after freeze drying was achieved in comparison with fluidization bed drying. Finally, the industrial processing of probiotic strains into the solid dosage form was studied. Tablets should be produced at hardness between 70 and 90 N and water activity of tablet mixture can be maintained below 0.3. Consequently, the drying step of the tablets in a hermetically closed space with at least 10 % of silica gel must be ensured. Thereafter, the tablets contain (5.4 ± 0.7)109 viable cells after 6 months of drying process. Capsule production technology has no significant effect on the cell‘s viability during production. The triplex blistering foil for primary blistering of probiotic capsules was chosen. The triplex foil, which has low values of water vapour transition rate (0.07 g H2O / (m2 × day) and oxygen transition rate (0.01 cm3/m2 × day), was chosen. Other studied blistering foils commonly used in the pharmaceutical industry are not suitable for long storage of solid dosage forms contained probiotics.
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Technology of production of fermented meat products
BEDRNÍČEK, Jan
The theme of this thesis is technology of production of fermented meat products and is focused on Lovecky salami. In the literature review, the work deals with the history, legislation, materials and technologies for the production of this group of meat products. There are also described methods of sensory analyse. Practical part dealt with: a) measuring of selected physical and chemical quantities (pH, water activity) during rippening and long-term storaging of Lovecky salami; b) influence of packaging method (unpacked, modified atmosphere, vacuum) on sensory value during long-term storaging. These results provide information about the ripening of Lovecky salami and cunsumer preferences in terms of packaging method. Experiment was cunducted in company MasoWest s.r.o. which starts with production of Lovecky salami.
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Quality parameters of dry fermented sausages
Pavlík, Zdeněk ; Ing.Miroslav Jůzl, Ph.D. (referee) ; Alena,, Saláková (advisor)
The aim of this study was to evaluate quality parameters of dry fermented sausages. Samples of two types of dry fermented sausages were sampled – sausage “Poličan“ and “Lovecký salám“, from three different producers. Basic physical-chemical analysis of quality parameters were performed in sausage mixture and immediately after end of ripening (21 days for Poličan and 16 days for Lovecký salám). Results of quality parameters analysis were compared with requirements of Czech legislation and differences between producers were observed. There were significant differences between producers, however all of them met requirements set by 326/2001 Czech notification as amended of Ministry of agricultural.
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Moulds and residential buildings
Dvořák, Tomáš ; Trachtová, Štěpánka (referee) ; Omelková, Jiřina (advisor)
Bachelor’s thesis is aimed at moulds and residential buildings. At moulds occurrence and at conditions for their growth and reproduction. Thesis defined their distribution, morphology, metabolism and type of reproduction. Describe most important genera of moulds, their identification and procedure during their elimination. Practical part is aimed at two methods of determination in two different types of residential building, in prefab house and in single family house. The object of practical part was to determinate the genus of occurring moulds
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